| Branch Name | First Stop | Start Time | Last Stop | End Time | Travel Time |
|---|
|
| ↓South to Union Station | Spadina Station Streetcar Platform | 1:25:00AM (following day) | Union Station Streetcar Platform | 1:50:00AM | 25:00 |
| ↑North to Spadina Station | Union Station Streetcar Platform | 1:32:00AM (following day) | Spadina Station Streetcar Platform | 1:57:00AM | 25:00 |
| ↑North to Queen | Union Station Streetcar Platform | 1:42:00AM (following day) | Richmond St West on Spadina Ave | 1:55:16AM | 13:16 |
| ↓South to Union Station | Spadina Station Streetcar Platform | 1:45:00AM (following day) | Union Station Streetcar Platform | 2:10:00AM | 25:00 |
| ↑North to Spadina Station | Union Station Streetcar Platform | 1:50:00AM (following day) | Spadina Station Streetcar Platform | 2:15:00AM | 25:00 |
| ↓South to Queen | Spadina Station Streetcar Platform | 1:55:00AM (following day) | Sullivan St on Spadina Ave | 2:03:43AM | 8:43 |
| ↓South to Queen | Spadina Station Streetcar Platform | 2:00:00AM (following day) | Sullivan St on Spadina Ave | 2:08:43AM | 8:43 |
| ↓South to Union Station | Spadina Station Streetcar Platform | 2:05:00AM (following day) | Union Station Streetcar Platform | 2:30:00AM | 25:00 |
| ↑North to Spadina Station | Union Station Streetcar Platform | 2:10:00AM (following day) | Spadina Station Streetcar Platform | 2:35:00AM | 25:00 |
| ↓South to Union Station | Spadina Station Streetcar Platform | 2:25:00AM (following day) | Union Station Streetcar Platform | 2:50:00AM | 25:00 |
| ↑North to Spadina Station | Union Station Streetcar Platform | 2:30:00AM (following day) | Spadina Station Streetcar Platform | 2:55:00AM | 25:00 |
| ↓South to Union Station | Spadina Station Streetcar Platform | 2:45:00AM (following day) | Union Station Streetcar Platform | 3:10:00AM | 25:00 |
| ↑North to Spadina Station | Union Station Streetcar Platform | 2:50:00AM (following day) | Spadina Station Streetcar Platform | 3:15:00AM | 25:00 |
| ↓South to Union Station | Spadina Station Streetcar Platform | 3:05:00AM (following day) | Union Station Streetcar Platform | 3:30:00AM | 25:00 |
| ↑North to Spadina Station | Union Station Streetcar Platform | 3:10:00AM (following day) | Spadina Station Streetcar Platform | 3:35:00AM | 25:00 |
| ↓South to Union Station | Spadina Station Streetcar Platform | 3:25:00AM (following day) | Union Station Streetcar Platform | 3:50:00AM | 25:00 |
| ↑North to Spadina Station | Union Station Streetcar Platform | 3:30:00AM (following day) | Spadina Station Streetcar Platform | 3:55:00AM | 25:00 |
| ↓South to Union Station | Spadina Station Streetcar Platform | 3:45:00AM (following day) | Union Station Streetcar Platform | 4:10:00AM | 25:00 |
| ↑North to Spadina Station | Union Station Streetcar Platform | 3:50:00AM (following day) | Spadina Station Streetcar Platform | 4:15:00AM | 25:00 |
| ↓South to Union Station | Spadina Station Streetcar Platform | 4:05:00AM (following day) | Union Station Streetcar Platform | 4:30:00AM | 25:00 |
| ↑North to Spadina Station | Union Station Streetcar Platform | 4:10:00AM (following day) | Spadina Station Streetcar Platform | 4:35:00AM | 25:00 |
| ↓South to Union Station | Spadina Station Streetcar Platform | 4:25:00AM (following day) | Union Station Streetcar Platform | 4:50:00AM | 25:00 |
| ↑North to Spadina Station | Union Station Streetcar Platform | 4:30:00AM (following day) | Spadina Station Streetcar Platform | 4:55:00AM | 25:00 |
| ↓South to Union Station | Spadina Station Streetcar Platform | 4:45:00AM (following day) | Union Station Streetcar Platform | 5:10:00AM | 25:00 |
| ↑North to Spadina Station | Union Station Streetcar Platform | 4:50:00AM (following day) | Spadina Station Streetcar Platform | 5:15:00AM | 25:00 |
| ↓South to Queen | Spadina Station Streetcar Platform | 4:55:00AM (following day) | Sullivan St on Spadina Ave | 5:03:43AM | 8:43 |
| ↑North to Spadina Station | Union Station Streetcar Platform | 5:10:00AM (following day) | Spadina Station Streetcar Platform | 5:35:00AM | 25:00 |
| ↓South to Queen | Spadina Station Streetcar Platform | 5:15:00AM (following day) | Sullivan St on Spadina Ave | 5:23:43AM | 8:43 |
| ↓South to Queen | Spadina Station Streetcar Platform | 5:35:00AM (following day) | Sullivan St on Spadina Ave | 5:43:43AM | 8:43 |